Immunohistochemical Detection of Intermediate Filament Nestin
نویسندگان
چکیده
منابع مشابه
Immunohistochemical detection of nestin in pediatric brain tumors.
Nestin is an intermediate filament protein (IFP) expressed in undifferentiated cells during CNS development and in CNS tumors. Previous studies have arrived at different conclusions in terms of which types of CNS tumors express nestin. In this report we establish an immunohistochemical protocol using antigen retrieval, which significantly enhances staining with two polyclonal anti-nestin antise...
متن کاملExpression of intermediate filament nestin in blood vessels of neural and non-neural tissues.
Our previous findings performed in rat tissues demonstrated that intermediate filament nestin is expressed in endothelial cells of newly formed blood vessels of developing organs and neural transplants. The aim of the present study was to identify other cellular markers expressed in nestin-positive (nestin+) blood vessels. To reach this goal we performed double immunofluorescent study to co-loc...
متن کاملRegulation of the intermediate filament protein nestin at rodent neuromuscular junctions by innervation and activity.
The intermediate filament nestin is localized postsynaptically at rodent neuromuscular junctions. The protein forms a filamentous network beneath and between the synaptic gutters, surrounds myofiber nuclei, and is associated with Z-discs adjacent to the junction. In situ hybridization shows that nestin mRNA is synthesized selectively by synaptic myonuclei. Although weak immunoreactivity is pres...
متن کاملTransient expression of the intermediate filament nestin during skeletal muscle development.
It has previously been established that skeletal muscle development is accompanied by changes in the composition of intermediate filaments: vimentin is expressed predominantly in myoblasts and desmin in adult myotubes. We show that the intermediate filament transitions during muscle development are more complex, and involve a transient expression of the recently discovered intermediate filament...
متن کاملExpression of nestin--a stem cell associated intermediate filament in human CNS tumours.
BACKGROUND & OBJECTIVES Nestin is an intermediate filament protein expressed in undifferentiated cells during the development of brain and is considered as a marker for neuroepithelial stem cells. Expression of this protein in various CNS tumour cells suggests the possibility of existence of tumour stem cell modulating the evolution. We carried out an immunohistochemical study to demonstrate th...
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ژورنال
عنوان ژورنال: Acta Medica (Hradec Kralove, Czech Republic)
سال: 1998
ISSN: 1211-4286,1805-9694
DOI: 10.14712/18059694.2019.168